Chen Shaohua, Zha Xianfeng, Yang Lijian, Li Bo, Liye Zhong, Li Yangqiu
Institute of Hematology, Medical College, Jinan University, Guangzhou, China.
Hematology. 2011 Jan;16(1):31-6. doi: 10.1179/102453311X12902908411832.
In order to elucidate the feature of T-cell receptor (TCR) signal transduction in T-cells from acute myeloid leukemia (AML), the expression levels of CD3gamma, delta, epsilon and zeta chain genes in CD3+ T cells were analyzed using real-time PCR. CD3+ T cells sorted from peripheral blood of 10 AML patients and 10 healthy donors were used in the study. Significantly lower expression levels of all four CD3gamma, delta, epsilon, and zeta chain genes were found in the AML samples. The expression pattern of the four CD3 chains was epsilon>gamma>delta>zeta in CD3+ T cells from AML samples, which was different from the healthy control group. In conclusion, the results provide a global gene expression profile of CD3gamma, delta, epsilon, and zeta chains in AML patients. Deficiency of all four CD3 gene expression levels might represent the feature related to T-cell immunodeficiency.
为了阐明急性髓系白血病（AML）患者T细胞中T细胞受体（TCR）信号转导的特征，采用实时定量PCR分析了CD3 + T细胞中CD3γ、δ、ε和ζ链基因的表达水平。研究使用了从10例AML患者和10名健康供者外周血中分选的CD3 + T细胞。在AML样本中发现所有四个CD3γ、δ、ε和ζ链基因的表达水平均显著降低。AML样本的CD3 + T细胞中四条CD3链的表达模式为ε>γ>δ>ζ，这与健康对照组不同。总之，这些结果提供了AML患者CD3γ、δ、ε和ζ链的整体基因表达谱。所有四个CD3基因表达水平的缺乏可能代表与T细胞免疫缺陷相关的特征。
