Ren Changhong, Gao Mingqing, Dornbos David, Ding Yuchuan, Zeng Xianwei, Luo Yumin, Ji Xunming
Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.
Neurol Res. 2011 Jun;33(5):514-9. doi: 10.1179/016164111X13007856084241.
To determine the protective effects of remote post-conditioning on ischemic brain lesions caused by middle cerebral artery (MCA) occlusion in rats.
A total of 54 animals were used in this present study. An ischemic stroke model was generated by 90-minute occlusion of right MCA (n = 42). Twelve rats were used as control for studying edema and blood-brain barrier (BBB) integrity. Remote post-conditioning was conducted immediately after MCA occlusion in the bilateral lower limb by occluding and releasing the femoral artery for three cycles; each occlusion and release lasted for 10 minutes. After 24 hours of reperfusion, the cerebral infarct volumes were quantified by 2,3,4-triphenytetrazolium-chloride, brain water content was determined by dry/wet weight method, and damage to the BBB was determined by Evans blue extravasation.
Remote post-conditioning significantly reduced brain infarct damage (P<0.0001). Brain edema was significantly (P<0.01) reduced after stroke in the remote post-conditioning group. BBB leakage was significantly reduced in the remote post-conditioning group when compared to the control ischemic groups (P<0.05).
These results provide evidence that remote post-conditioning, which was initiated after ischemia and before reperfusion, protects against brain injury in experimental ischemic stroke.
确定远程后适应对大鼠大脑中动脉（MCA）闭塞所致缺血性脑损伤的保护作用。
本研究共使用54只动物。通过闭塞右侧MCA 90分钟建立缺血性卒中模型（n = 42）。12只大鼠用作研究水肿和血脑屏障（BBB）完整性的对照。在MCA闭塞后立即对双侧下肢进行远程后适应，通过阻断和松开股动脉3个循环；每次阻断和松开持续10分钟。再灌注24小时后，用2,３,４-三苯基氯化四氮唑定量脑梗死体积，用干/湿重法测定脑含水量，用伊文思蓝外渗法测定BBB损伤情况。
远程后适应显著减轻脑梗死损伤（P<0.0001）。远程后适应组卒中后脑水肿显著减轻（P<0.01）。与对照缺血组相比，远程后适应组BBB渗漏显著减少（P<0.05）。
这些结果证明，在缺血后再灌注前启动的远程后适应可保护实验性缺血性卒中的脑损伤。
