Li M G, Starlinger P
Institut für Genetik, Universität zu Köln, Cologne, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6044-8. doi: 10.1073/pnas.87.16.6044.
Mutations of transposable element Ac were tested for their capability to excise themselves from their location autonomously, to be excised by an active Ac, or to act in trans in the excision of an Ac delta element. Removal of 101 amino acids from the N terminus of the Ac protein does not decrease excision. A cis-acting site between base pairs 186 and 207 is important for excision by the wild-type protein but is not necessary for excision by the truncated protein. Improvement of the sequence context of the first AUG does not have a significant effect. Mutations in a small open reading frame of Ac encoding a 102-amino acid protein do not visibly alter excision frequency.
对转座元件Ac的突变体进行了测试，以检验它们自主从其所在位置切除自身的能力、被活性Ac切除的能力，或在切除Acδ元件时进行反式作用的能力。从Ac蛋白的N端去除101个氨基酸不会降低切除效率。野生型蛋白切除时，碱基对186和207之间的顺式作用位点很重要，但截短蛋白切除时该位点并非必需。起始AUG序列上下文的改善没有显著影响。Ac中一个编码102个氨基酸蛋白的小开放阅读框中的突变不会明显改变切除频率。
