玉米En/Spm转座元件的切除需要两种由元件编码的蛋白质。
Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
作者信息
Frey M, Reinecke J, Grant S, Saedler H, Gierl A
机构信息
Max-Planck-Institut für Züchtungsforschung, Molekulare Pflanzengenetik, Köln, FRG.
出版信息
EMBO J. 1990 Dec;9(12):4037-44. doi: 10.1002/j.1460-2075.1990.tb07625.x.
Abstract
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.
摘要
在转基因烟草中开发了一种用于En/Spm的切除检测系统。其切除和整合特性与玉米的天然系统相似。在这个转基因模型系统中，切除需要两种由En/Spm编码的反式作用功能，即TNPA和TNPD。提出了一种转座的生化模型，该模型可能也适用于其他转座元件。
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