Department of Biochemistry, University of Stellenbosch, Stellenbosch, Private Bag X1, Matieland 7602, South Africa;
Am J Bot. 2001 Nov;88(11):2088-100.
Sequence data from the intron and spacer of the trnL-F chloroplast region elucidate the phylogenetic relationships of the tribe Diseae (Orchidoideae: Orchidaceae). Within Diseae, 41 species of Disa, two of Brownleea, three of Satyrium, and two of Corycium were included, with five species of Habenaria sensu lato (Orchideae) and one epidendroid as outgroups. The sequences revealed substitutions and considerable length variation, due mainly to the presence of repeat motifs. Phylogenetic analysis using parsimony revealed five distinct clades. The branching order of the five weakly supported the paraphyly of Diseae, with the successive divergence of Brownleea, Corycium, Habenaria, Satyrium, and Disa. Within the monophyletic Disa, three main groupings appeared, two strongly supported clades representing sect. Racemosae and sect. Coryphaea and the third grouping containing several clades currently grouped into sections based on morphological phylogenies. Some discrepancies between the molecular phylogeny and the phylogeny based on morphological characters may require reevaluation of some of the morphological characters. The presence of different numbers of repeat motifs, both among different taxa and within taxa, indicates that these characters may be phylogenetically informative at the population level.
来自 trnL-F 叶绿体区内含子和间隔区的序列数据阐明了 Diseae（Orchidoideae：Orchidaceae）族的系统发育关系。在 Diseae 中，包括 41 种 Disa、2 种 Brownleea、3 种 Satyrium 和 2 种 Corycium，以及 5 种广义 Habenaria（Orchideae）和 1 种 Epidendroid 作为外群。序列揭示了替换和相当大的长度变异，主要是由于重复基序的存在。使用简约法进行的系统发育分析显示出五个不同的分支。五个分支的顺序较弱地支持 Diseae 的并系性，其中 Brownleea、Corycium、Habenaria、Satyrium 和 Disa 依次分化。在单系的 Disa 中，出现了三个主要的分组，两个强烈支持的分支代表 sect. Racemosae 和 sect. Coryphaea，第三个分组包含几个目前根据形态系统发育分组到各个节的分支。分子系统发育与基于形态特征的系统发育之间的一些差异可能需要重新评估一些形态特征。不同分类群之间以及同一分类群内重复基序数量的不同表明，这些特征在种群水平上可能具有系统发育信息。
