The Lewis B. and Dorothy Cullman Program for Molecular Systematics Studies, The New York Botanical Garden, Bronx, New York 10458-5126;
Am J Bot. 1999 Feb;86(2):208-24.
Cladistic parsimony analyses of rbcL nucleotide sequence data from 171 taxa representing nearly all tribes and subtribes of Orchidaceae are presented here. These analyses divide the family into five primary monophyletic clades: apostasioid, cypripedioid, vanilloid, orchidoid, and epidendroid orchids, arranged in that order. These clades, with the exception of the vanilloids, essentially correspond to currently recognized subfamilies. A distinct subfamily, based upon tribe Vanilleae, is supported for Vanilla and its allies. The general tree topology is, for the most part, congruent with previously published hypotheses of intrafamilial relationships; however, there is no evidence supporting the previously recognized subfamilies Spiranthoideae, Neottioideae, or Vandoideae. Subfamily Spiranthoideae is embedded within a single clade containing members of Orchidoideae and sister to tribe Diurideae. Genera representing tribe Tropideae are placed within the epidendroid clade. Most traditional subtribal units are supported within each clade, but few tribes, as currently circumscribed, are monophyletic. Although powerful in assessing monophyly of clades within the family, in this case rbcL fails to provide strong support for the interrelationships of the subfamilies (i.e., along the spine of the tree). The cladograms presented here should serve as a standard to which future morphological and molecular studies can be compared.
这里呈现了对来自 171 个分类单元的 rbcL 核苷酸序列数据进行分支系统学简约分析的结果，这些分类单元代表了兰科几乎所有的族和亚族。这些分析将兰科分为五个主要的单系分支：兜兰亚科、杓兰亚科、鸟巢兰亚科、兰亚科和万代兰亚科，其排列顺序与分支系统树上的顺序一致。除了鸟巢兰亚科之外，这些分支基本上对应于当前公认的亚科。以族 Vanilleae 为基础的一个独特的亚科得到了支持，用于包括香子兰及其近缘属。总的树拓扑结构在很大程度上与先前发表的兰科内部关系假说一致；然而，没有证据支持先前公认的亚科 Spiranthoideae、Neottioideae 或 Vandoideae。Spiranthoideae 亚科嵌入一个包含 Orchidoideae 成员的分支中，与 Diurideae 族互为姐妹群。代表 Tropideae 族的属被放置在 epidendroid 分支中。大多数传统的亚族单位在每个分支中都得到了支持，但很少有像目前定义的那样，是单系的。尽管 rbcL 在评估科内分支的单系性方面非常强大，但在这种情况下，它无法为亚科的相互关系（即树的主干）提供强有力的支持。这里呈现的系统发育树应该作为未来形态学和分子研究的标准，可以与之进行比较。
