López-Guerrero J A, Pimentel-Muiños F X, Fresno M, Alonso M A
Centro de Biología Molecular, Universidad Autónoma, CSIC, Madrid, Spain.
Virus Res. 1990 Jun;16(2):225-30. doi: 10.1016/0168-1702(90)90025-7.
Poliovirus infection of monocytic U937 cells is characterized by an incomplete shut-off of host protein synthesis occurring at late times postinfection and by a slow accumulation of viral mRNA which is not efficiently translated. By using exogenous cytokines, neutralizing antibodies to different cytokines and specific cDNA probes, we have studied the involvement of soluble factors on the restricted replication of poliovirus in U937 cells. Our results show that both IFN-gamma and TNF-alpha mRNA levels increased in U937 cells upon poliovirus infection. Moreover, treatment of infected U937 cells with antibodies to IFN-gamma induced a remarkably stronger shut-off of host protein synthesis than that found in control cultures. A role of IFN-gamma in protecting the infected cells from an extensive blockade of host mRNA translation is proposed.
脊髓灰质炎病毒感染单核细胞U937细胞的特征是在感染后期宿主蛋白质合成不完全关闭，以及病毒mRNA缓慢积累且不能有效翻译。通过使用外源性细胞因子、针对不同细胞因子的中和抗体和特异性cDNA探针，我们研究了可溶性因子在脊髓灰质炎病毒在U937细胞中受限复制中的作用。我们的结果表明，脊髓灰质炎病毒感染后，U937细胞中IFN-γ和TNF-α的mRNA水平均升高。此外，用抗IFN-γ抗体处理感染的U937细胞，导致宿主蛋白质合成的关闭比对照培养物中更为明显。有人提出IFN-γ在保护感染细胞免受宿主mRNA翻译广泛阻断方面发挥作用。
