Friedrich A, Kleim J P, Schneweis K E
Institute of Medical Microbiology and Immunology, University of Bonn, Federal Republic of Germany.
Arch Virol. 1990;113(1-2):107-13. doi: 10.1007/BF01318359.
Latency of thymidine kinase-negative mutants of herpes simplex virus (TK- HSV) could not be detected by reactivating the virus from the ganglia of infected mice. Because Southern blot hybridization was not sensitive enough to detect viral DNA, positive results obtained by dot blot hybridization were ascertained by the highly specific and sensitive polymerase chain reaction (PCR), which detected both latent TK- HSV type 1 and 2 DNA from the trigeminal ganglia of infected mice.
通过从感染小鼠的神经节中重新激活病毒，无法检测到单纯疱疹病毒胸苷激酶阴性突变体（TK-HSV）的潜伏情况。由于Southern印迹杂交检测病毒DNA的灵敏度不够，通过斑点印迹杂交获得的阳性结果用高度特异且灵敏的聚合酶链反应（PCR）来确定，该反应能检测到来自感染小鼠三叉神经节的潜伏性1型和2型TK-HSV DNA。
