Schneweis K E, Forstbauer H, Olbrich M, Tag M
Med Microbiol Immunol. 1984;173(4):187-96. doi: 10.1007/BF02122110.
This study was undertaken to establish the role of virulence of various herpes simplex virus (HSV) strains in the course of infection when applying the virus to the non-injured mucous membranes of mice. Wild-type HSV-type 1 (HSV-1) strains with marked differences in their neurovirulence following intracerebral inoculation showed minor differences in virulence after vaginal inoculation, but essentially their neurovirulence in cerebral infection corresponded to their virulence on the mucous membranes. In comparison with the wild-types, however, there were pronounced differences among syn- and TK--mutants of HSV-1 and HSV-2 in the degree of virulence at different sites in the course of virus infection. Whereas syn-mutants proved avirulent on the mucous membranes but not in neural tissues, TK--mutants were avirulent both on mucous membranes and in neural tissues. Ts-mutants of HSV-2 were not found to establish themselves when administered to the non-injured mucous membranes, nor did they induce neutralizing antibodies, but a later challenge with the wild-type virus at the same site lead only to an attenuated course of infection.
本研究旨在确定当将各种单纯疱疹病毒（HSV）毒株应用于小鼠未受损的粘膜时，其毒力在感染过程中的作用。脑内接种后神经毒力有显著差异的野生型1型单纯疱疹病毒（HSV-1）毒株，经阴道接种后的毒力差异较小，但基本上它们在脑内感染的神经毒力与在粘膜上的毒力相对应。然而，与野生型相比，HSV-1和HSV-2的同步和TK-突变体在病毒感染过程中不同部位的毒力程度存在明显差异。同步突变体在粘膜上无毒力，但在神经组织中有毒力，而TK-突变体在粘膜和神经组织中均无毒力。HSV-2的温度敏感突变体在应用于未受损的粘膜时未发现能够定植，也未诱导产生中和抗体，但随后在同一部位用野生型病毒进行攻击时，仅导致感染过程减弱。
