Kuchel P W, Berthon H A, Bubb W A, McIntyre L M, Nygh N K, Thorburn D R
Department of Biochemistry, University of Sydney, NSW, Australia.
Biomed Biochim Acta. 1990;49(2-3):S105-10.
31P NMR was used to monitor the concentrations of some of the intermediates of the non-oxidative pentose phosphate pathway (PPP) during the dissimilation of inosine and phosphate in dilute haemolysates. The temperature dependence of the ketone/hydrate ratio in dihydroxyacetone phosphate and the relative proportions of the isomers of sedoheptulose 1,7-bisphosphate were measured. The 31P NMR time courses were compared with the simulations obtained with a computer model of the modified F-type PPP.
采用³¹P核磁共振技术监测稀溶血产物中肌苷和磷酸盐异化过程中非氧化戊糖磷酸途径(PPP)某些中间产物的浓度。测定了磷酸二羟丙酮中酮/水合物比例的温度依赖性以及景天庚酮糖1,7-二磷酸异构体的相对比例。将³¹P核磁共振时间进程与用改良F型PPP计算机模型获得的模拟结果进行了比较。