School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
Theor Appl Genet. 2011 Sep;123(5):803-13. doi: 10.1007/s00122-011-1628-2. Epub 2011 Jun 14.
Pea rust caused by Uromyces fabae (Pers.) de-Bary is a major problem in warm humid regions causing huge economic losses. A mapping population of 136 F(6:7) recombinant inbred lines (RILs) derived from the cross between pea genotypes, HUVP 1 (susceptible) and FC 1 (resistant) was evaluated in polyhouse as well as under field conditions during two consecutive years. Infection frequency (IF) and area under disease progress curve (AUDPC) were used for evaluation of rust reaction of the RILs. A linkage map was constructed with 57 polymorphic loci selected from 148 simple sequence repeats (SSRs), 3 sequence tagged sites (STS), and 2 random amplified polymorphic (RAPD) markers covering 634 cM of genetic distance on the seven linkage groups of pea with an average interval length of 11.3 cM. Composite interval mapping (CIM) revealed one major (Qruf) and one minor (Qruf1) QTL for rust resistance on LGVII. The LOD (5.2-15.8) peak for Qruf was flanked by SSR markers, AA505 and AA446 (10.8 cM), explaining 22.2-42.4% and 23.5-58.8% of the total phenotypic variation for IF and AUDPC, respectively. The minor QTL was environment-specific, and it was detected only in the polyhouse (LOD values 4.2 and 4.8). It was flanked by SSR markers, AD146 and AA416 (7.3 cM), and explained 11.2-12.4% of the total phenotypic variation. The major QTL Qruf was consistently identified across all the four environments. Therefore, the SSR markers flanking Qruf would be useful for marker-assisted selection for pea rust (U. fabae) resistance.
豌豆锈病由菜豆单胞锈菌（Uromyces fabae）引起，在温暖潮湿的地区是一个主要问题，会造成巨大的经济损失。在两个连续的年份里，在温室和田间条件下，对来自豌豆基因型 HUVP 1（感病）和 FC 1（抗病）杂交的 136 个 F(6:7)重组自交系（RILs）的作图群体进行了评估。使用感染频率（IF）和病害进展曲线下的面积（AUDPC）来评估 RILs 的锈病反应。利用从 148 个简单序列重复（SSR）、3 个序列标记位点（STS）和 2 个随机扩增多态性（RAPD）标记中选择的 57 个多态性标记，构建了一个包含豌豆 7 个连锁群的连锁图谱，遗传距离为 634cM，平均间隔长度为 11.3cM。复合区间作图（CIM）在 LGVII 上发现了一个主要（Qruf）和一个次要（Qruf1）的锈病抗性 QTL。Qruf 的 LOD（5.2-15.8）峰值被 SSR 标记 AA505 和 AA446 侧翼（10.8cM），分别解释了 IF 和 AUDPC 总表型变异的 22.2-42.4%和 23.5-58.8%。次要 QTL 是特定于环境的，仅在温室中检测到（LOD 值为 4.2 和 4.8）。它被 SSR 标记 AD146 和 AA416 侧翼（7.3cM），解释了总表型变异的 11.2-12.4%。主要 QTL Qruf 在所有四个环境中都得到了一致的鉴定。因此，侧翼 Qruf 的 SSR 标记将有助于对豌豆锈病（U. fabae）抗性进行标记辅助选择。
