Dezzutti C S, Frazier D E, Huff L Y, Stromberg P C, Olsen R G
Center for Retrovirus Research, Columbus, Ohio 43210.
Cancer Res. 1990 Sep 1;50(17 Suppl):5687S-5691S.
Five macaques received two vaccinations consisting of soluble human T-cell lymphotropic virus type I proteins from a cell/serum-free human T-cell lymphotropic virus type I-producing cell line. Five other macaques were vaccine controls. All were challenged with a simian T-cell lymphotropic virus type I-producing cell line. The vaccinated macaques generated a strong serological response to challenge as opposed to the control macaques. Western blot analysis of the sera showed that both groups recognized gag and env proteins, but the vaccinate's sera reacted better to the env proteins. Additionally, the antibody produced by both groups had antibody-dependent, complement-mediated cytotoxic activity toward both human and simian T-cell lymphotropic virus type I-infected target cells. The responses of lymphocytes and neutrophils, as measured by lymphocyte blast transformation and chemiluminescence response, respectively, showed no apparent difference between the vaccinates and controls. Testing for reverse transcriptase in lymphocyte supernatants revealed that the controls contained reverse transcriptase activity, while the vaccinates remained negative. The data presented here demonstrate that the vaccine was successful in protecting Macaca nemestrina from simian T-cell lymphotropic virus type I infection.
五只猕猴接受了两次疫苗接种，疫苗由来自无细胞/无血清的产I型人嗜T细胞病毒细胞系的可溶性I型人嗜T细胞病毒蛋白组成。另外五只猕猴作为疫苗对照。所有猕猴均用产I型猿猴嗜T细胞病毒细胞系进行攻击。与对照猕猴相比，接种疫苗的猕猴在受到攻击后产生了强烈的血清学反应。血清的蛋白质印迹分析表明，两组都识别gag和env蛋白，但接种疫苗的猕猴血清对env蛋白的反应更好。此外，两组产生的抗体对人和I型猿猴嗜T细胞病毒感染的靶细胞均具有抗体依赖性、补体介导的细胞毒性活性。分别通过淋巴细胞增殖转化和化学发光反应测量的淋巴细胞和中性粒细胞反应在接种疫苗的猕猴和对照之间没有明显差异。淋巴细胞上清液中的逆转录酶检测显示，对照含有逆转录酶活性，而接种疫苗的猕猴仍为阴性。此处提供的数据表明，该疫苗成功地保护了豚尾猕猴免受I型猿猴嗜T细胞病毒感染。
