Johansson M H, Deinum J, Marklund S L, Sjöquist P O
Department of Cardiovascular Pharmacology, AB Hässle, Mölndal, Sweden.
Cardiovasc Res. 1990 Jun;24(6):500-3. doi: 10.1093/cvr/24.6.500.
The aim of the study was to determine whether recombinant human extracellular superoxide dismutase type C (rh-EC-SOD C) has antioxidant effects similar to those of the previously studied Cu-Zn superoxide dismutase.
Electron paramagnetic resonance spectroscopy was used for quantification of free radical generation during reperfusion of postischaemic isolated rat hearts. The hearts were perfused with Krebs-Henseleit buffer and then subjected to 15 min global ischaemia. N-tert-butyl-alpha-phenylnitrone (PBN) was added as a spin trapping agent to the perfusate.
18 hearts from Sprague-Dawley rats (280-400 g) were used.
Reperfusion resulted in a burst of radical formation. In presence of rh-EC-SOD C plus catalase, the PBN spin adduct concentration was only 18% (p less than 0.05) of the value in the control group. With bovine Cu-Zn superoxide dismutase plus catalase added to the buffer the corresponding spin adduct formation was 44% of control (NS = v rh-EC-SOD C).
In isolated rat heart, rh-EC-SOD C reduces free radical concentrations at least to the same extent as Cu-Zn superoxide dismutase.
本研究旨在确定重组人C型细胞外超氧化物歧化酶（rh-EC-SOD C）是否具有与先前研究的铜锌超氧化物歧化酶相似的抗氧化作用。
采用电子顺磁共振光谱法对缺血后分离的大鼠心脏再灌注期间的自由基生成进行定量。心脏先用克雷布斯-亨泽莱特缓冲液灌注，然后进行15分钟全心缺血。将N-叔丁基-α-苯基硝酮（PBN）作为自旋捕获剂添加到灌注液中。
使用了18只来自斯普拉格-道利大鼠（体重280-400克）的心脏。
再灌注导致自由基形成爆发。在rh-EC-SOD C加过氧化氢酶存在的情况下，PBN自旋加合物浓度仅为对照组的18%（p<0.05）。在缓冲液中添加牛铜锌超氧化物歧化酶加过氧化氢酶时，相应的自旋加合物形成量为对照组的44%（无显著性差异=与rh-EC-SOD C相比）。
在离体大鼠心脏中，rh-EC-SOD C降低自由基浓度的程度至少与铜锌超氧化物歧化酶相同。
