Department of Pediatric Hematology, Oncology and Clinical Immunology, Heinrich Heine University, Duesseldorf, Germany.
Scand J Immunol. 2011 Oct;74(4):406-11. doi: 10.1111/j.1365-3083.2011.02591.x.
Human invariant natural killer T cells (iNKT cells) are a unique population of T cells that express a semi-invariantly rearranged T cell receptor (TCR) and are involved in a variety of immunoregulatory processes. We assessed the frequency of peripheral blood iNKT cells in 64 healthy Caucasian children from 7 months to 18 years of age and five cord blood samples by flow cytometry. iNKT cells were measured as CD3(+) cells co-expressing TCRVα24 and TCRVβ11 and using the monoclonal antibody 6B11, which recognizes specifically their invariant TCR rearrangement. The absolute number of iNKT cells ranged from 86 to 10,499 (CD3(+) /TCRVα24(+) / TCRVβ11(+)) and 233 to 11,167 (CD3(+) /6B11(+)) iNKT cells per millilitre of blood. This range is stable from birth to adulthood. The relative iNKT cell count was found to be 0.003-0.71% (CD3(+) /TCRVα24/TCRVβ11) and 0.019-0.776% (CD3/6B11) of peripheral blood T cells and shows only a slight increase with age.
人类不变自然杀伤 T 细胞（iNKT 细胞）是一种独特的 T 细胞群体，表达半不变重排的 T 细胞受体（TCR），并参与多种免疫调节过程。我们通过流式细胞术评估了 64 名健康白种人儿童（7 个月至 18 岁）和 5 份脐带血样本外周血 iNKT 细胞的频率。iNKT 细胞被定义为共表达 TCRVα24 和 TCRVβ11 的 CD3(+)细胞，并使用单克隆抗体 6B11 进行测量，该抗体特异性识别其不变的 TCR 重排。iNKT 细胞的绝对数量范围为每毫升血液 86 至 10,499（CD3(+) / TCRVα24(+) / TCRVβ11(+)）和 233 至 11,167（CD3(+) / 6B11(+））个 iNKT 细胞。这个范围从出生到成年期是稳定的。相对 iNKT 细胞计数为外周血 T 细胞的 0.003-0.71%（CD3(+) / TCRVα24/TCRVβ11）和 0.019-0.776%（CD3/6B11），并仅随年龄略有增加。
