Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic.
J Appl Microbiol. 2011 Sep;111(3):537-46. doi: 10.1111/j.1365-2672.2011.05073.x. Epub 2011 Jul 4.
To increase rumen output of γ-linolenic acid (GLA), we used two cereal-derived substrates, ground barley (GB) and rye bran (RB), enriched with fungal GLA as components of feed rations. We examined their effects on rumen fermentation patterns, lipid metabolism and the ciliated protozoan population in an artificial rumen.
Four diets consisting of meadow hay (MH) plus unfermented (GB or RB) or prefermented (GB - TE or RB - TE) cereal-derived substrates were fermented in an artificial rumen with ovine rumen inoculum. The cereal-derived substrates were prefermented with the fungus Thamnidium elegans (TE) by fungal solid-state fermentation. The diets with TE increased the rumen input of dietary GLA (mg day(-1)) from 0 to 21 (GB - TE) or 26 (RB - TE). Both experimental diets increased the rumen output of GLA (P < 0.001). Adverse effects on the ciliate population were observed. Both diets also had an effect on the fatty acids profile. Fermentation patterns were also affected with MH + RB - TE.
Cereal-derived substrates enriched with GLA effectively enhanced the output of GLA in artificial rumen.
The ability of the fungal strain T. elegans to grow and utilize various agro-industrial substrates might be useful in developing potential new animal diets enriched in GLA.
为了增加瘤胃中 γ-亚麻酸（GLA）的产量，我们使用了两种谷物衍生底物，即粉碎的大麦（GB）和黑麦麸皮（RB），并将其作为饲料日粮的成分进行真菌 GLA 富集。我们在人工瘤胃中研究了它们对瘤胃发酵模式、脂类代谢和纤毛虫原虫种群的影响。
四种日粮由草地干草（MH）加未发酵（GB 或 RB）或预发酵（GB - TE 或 RB - TE）的谷物衍生底物组成，用绵羊瘤胃液接种物在人工瘤胃中发酵。谷物衍生底物用真菌 Thamnidium elegans（TE）进行真菌固态发酵预发酵。添加 TE 的日粮增加了瘤胃输入的膳食 GLA（mg 天(-1)），从 0 增加到 21（GB - TE）或 26（RB - TE）。两种实验日粮均增加了 GLA 的瘤胃输出（P < 0.001）。观察到对纤毛虫种群的不利影响。两种日粮还对脂肪酸谱有影响。MH + RB - TE 也影响了发酵模式。
用 GLA 富集的谷物衍生底物有效地提高了人工瘤胃中 GLA 的产量。
真菌菌株 T. elegans 生长和利用各种农业工业底物的能力可能有助于开发富含 GLA 的潜在新型动物日粮。
