Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovak Republic.
J Biotechnol. 2013 Oct 20;168(2):130-4. doi: 10.1016/j.jbiotec.2013.03.016. Epub 2013 Apr 9.
Four Mucor strains were tested for their ability to grow on four cereal substrates and enriched them with gamma-linolenic acid (GLA) and β-carotene. M. circinelloides CCF-2617 as the best producer accumulated of both GLA and β-carotene in high amounts during utilization of rye bran/spent malt grains (3:1). The first growth phase was characterized by rapid GLA biosynthesis, while distinct β-carotene formation was found in the stationary fungal growth. Therefore various cultivation conditions were tested in order to optimize the yield of either GLA or β-carotene. The fungus grown on cereal substrate supplemented with glucose produced maximal 8.5 mg β-carotene and 12.1 g GLA in 1 kg fermented substrate, respectively. On the other hand, the highest amount of GLA in the fermented substrate (24.2 g/kg) was achieved when 30% of sunflower oil was employed to the substrate. Interestingly, β-carotene biosynthesis was completely inhibited when either whey or linseed oil were added to the substrate.
四种毛霉菌株被测试了其在四种谷物基质上生长的能力，并在其中添加γ-亚麻酸（GLA）和β-胡萝卜素进行了富化。M. circinelloides CCF-2617 作为最好的生产者，在利用黑麦麸/废麦芽粒（3:1）时，大量积累了这两种物质。第一个生长阶段的特点是 GLA 的快速生物合成，而在真菌静止生长阶段则发现了明显的β-胡萝卜素形成。因此，测试了各种培养条件，以优化 GLA 或β-胡萝卜素的产量。在添加葡萄糖的谷物基质上生长的真菌在 1 公斤发酵基质中分别产生了最大量的 8.5 毫克β-胡萝卜素和 12.1 克 GLA。另一方面，当向基质中添加 30%的葵花籽油时，发酵基质中 GLA 的含量达到最高（24.2 克/公斤）。有趣的是，当向基质中添加乳清或亚麻籽油时，β-胡萝卜素的生物合成完全被抑制。
