哇巴因不敏感的大鼠肾近端小管纯悬浮液中的钠-ATP酶活性

Ouabain-insensitive, Na-ATPase activity in pure suspensions of rat kidney proximal tubules.

作者信息

Marín R, Gómez D C, Rodríguez G A, Proverbio T, Proverbio F

机构信息

Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.

出版信息

FEBS Lett. 1990 Aug 20;269(1):77-8. doi: 10.1016/0014-5793(90)81122-5.

DOI:10.1016/0014-5793(90)81122-5

PMID:2167245

Abstract

The present work was undertaken to evaluate the distribution of the Na-ATPase activity in the different components of the rat kidney cortex. Suspensions of glomeruli, proximal and distal tubules were prepared following a collagenase digestion of outermost kidney cortex slices and a separation on a Percoll gradient. It was found that the Na-ATPase activity is higher in the fraction enriched in proximal tubules. The fraction enriched in glomeruli and in distal tubules show also a Na-ATPase activity, but it is lower.

摘要

本研究旨在评估大鼠肾皮质不同组分中钠-ATP酶活性的分布。通过对最外层肾皮质切片进行胶原酶消化，并在Percoll梯度上进行分离，制备了肾小球、近端小管和远端小管的悬浮液。结果发现，富含近端小管的组分中钠-ATP酶活性较高。富含肾小球和远端小管的组分也显示出钠-ATP酶活性，但较低。