State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Planta Med. 2011 Nov;77(17):1950-7. doi: 10.1055/s-0030-1271186. Epub 2011 Jun 14.
Cuscuta chinensis and Cuscuta australis, the two botanical sources of the Chinese herbal medicine Tu-Si-Zi, were distinguished from each other based on qualitative and quantitative chemical analysis. By HPLC‑DAD‑MS, a total of 36 compounds were characterized from these two Cuscuta species, including 14 flavonoids, 17 quinic acid derivatives, and 5 lignans. In addition, HPLC‑UV was applied to determine seven major compounds (6 flavonoids plus chlorogenic acid) in 27 batches of Tu-Si-Zi. The results revealed that the amounts of the three classes of compounds varied significantly between the species. C. australis contained more flavonoids but less quinic acid derivatives and lignans than C. chinensis. Particularly, the amounts of kaempferol and astragalin in C. australis were remarkably higher than in C. chinensis. This finding could be valuable for the quality control of Tu-Si-Zi.
菟丝子是一种中药材，其植物来源有两种，分别为中国菟丝子和南方菟丝子。本研究通过定性和定量化学分析的方法对这两种菟丝子进行区分。采用高效液相色谱-二极管阵列检测-质谱联用（HPLC-DAD-MS）技术，从这两种菟丝子中共鉴定出 36 种化合物，包括 14 种黄酮类化合物、17 种奎宁酸衍生物和 5 种木脂素。此外，还采用高效液相色谱-紫外检测（HPLC-UV）法测定了 27 批菟丝子中 7 种主要化合物（6 种黄酮类化合物加绿原酸）的含量。结果表明，这两个物种中这 3 类化合物的含量差异显著。与中国菟丝子相比，南方菟丝子含有更多的黄酮类化合物，但奎宁酸衍生物和木脂素含量较低。特别是，南方菟丝子中山柰酚和黄芪苷的含量明显高于中国菟丝子。该研究结果可为菟丝子的质量控制提供参考。
