Department of Anatomy, Histology and Embryology, Semmelweis University, Tüzoltó u. 58, Budapest, Hungary.
J Comp Neurol. 2012 Jan 1;520(1):100-16. doi: 10.1002/cne.22696.
To understand better the rate of neurogenesis and the distribution of new neurons in posthatch domestic chicks, we describe and compare the expression of the neuronal nuclei protein (NeuN, a.k.a. Fox-3) and doublecortin antigens in the whole brain of chicks 2 days, 8 days, and 14 weeks posthatch. In the forebrain ventricular and paraventricular zones, the density of bromodeoxyuridine-, NeuN-, and doublecortin-labeled cells was compared between chicks 24 hours and 7 days after an injection of bromodeoxyuridine (2 and 8 days posthatch, respectively). The distribution of NeuN-labeled neurons was similar to Nissl-stained tissue, with the exception of some areas where neurons did not express NeuN: cerebellar Purkinje cells and olfactory bulb mitral cells. The ventral tegmental area of 2-day-old chicks was also faintly labeled. The distribution of doublecortin was similar at all timepoints, with doublecortin-labeled profiles located throughout all forebrain areas as well as in the cerebellar granule cell layer. However, doublecortin labeling was not detectable in any midbrain or brainstem areas. Our data indicate that a significant number of new neurons is still formed in the telencephalon of posthatch domestic chicks, whereas subtelencephalic areas (except for the cerebellum) finish their neuronal expansion before hatching. Most newly formed cells in chicks leave the paraventricular zone after hatching, but a pool of neurons stays in the vicinity of the ventricular zone and matures in situ within 7 days. Proliferating cells often migrate laterally along forebrain laminae into still-developing brain areas.
为了更好地理解神经发生的速度和新生神经元在出壳后家鸡脑中的分布，我们描述并比较了神经元核蛋白（NeuN，又名 Fox-3）和双皮质素抗原在出壳后 2 天、8 天和 14 周龄家鸡全脑中的表达。在前脑脑室和室旁区，比较了溴脱氧尿苷、NeuN 和双皮质素标记细胞在出壳后 24 小时和 7 天（分别为 2 天和 8 天龄）注射溴脱氧尿苷后 2 天和 8 天龄的小鸡之间的密度。NeuN 标记神经元的分布与尼氏染色组织相似，除了一些不表达 NeuN 的区域：小脑浦肯野细胞和嗅球僧帽细胞。2 天大的小鸡腹侧被盖区也有微弱的标记。双皮质素的分布在所有时间点都相似，双皮质素标记的形态位于所有前脑区域以及小脑颗粒细胞层。然而，在中脑或脑干区域都无法检测到双皮质素标记。我们的数据表明，大量的新神经元仍在家鸡出壳后的端脑形成，而亚端脑区域（除了小脑）在出壳前完成了神经元的扩张。大多数新形成的细胞在出壳后离开室旁区，但在室周区附近仍有一小群神经元，在 7 天内成熟。增殖细胞通常沿前脑层侧向迁移到仍在发育的脑区。
