Department of Physiology, Federal University of Sergipe, São Cristóvão, Brazil.
Phytother Res. 2011 Aug;25(8):1236-41. doi: 10.1002/ptr.3550. Epub 2011 Jun 14.
In this study, we describe the antinociceptive activity of the ethanol extract (EE), chloroform (CF) and methanol (MF) fractions obtained from Sida cordifolia, popularly known in Brazil as "malva branca" or "malva branca sedosa". Leaves of S. cordifolia were used to produce the crude ethanol extract and after CF and MF. Experiments were conducted on Swiss mice using the glutamate and formalin-induced orofacial nociception. In the formalin test, all doses of EE, CF and MF significantly reduced the orofacial nociception in the first (p < 0.001) and second phase (p < 0.001), which was also naloxone-sensitive. In the glutamate-induced nociception test, only CF and MF significantly reduced the orofacial nociceptive behavior with inhibition percentage values of 48.1% (100 mg/kg, CF), 56.1% (200 mg/kg, CF), 66.4% (400 mg/kg, CF), 48.2 (200 mg/kg, MF) and 60.1 (400 mg/kg, MF). Furthermore, treatment of the animals with EE, CF and MF was not able to promote motor activity changes. These data demonstrate that S. cordifolia has a pronounced antinociceptive activity on orofacial nociception. However, pharmacological and chemical studies are necessary in order to characterize the responsible mechanisms for this antinociceptive action and also to identify other bioactive compounds present in S. cordifolia.
在这项研究中，我们描述了从巴西俗称“malva branca”或“malva branca sedosa”的 Sida cordifolia 中获得的乙醇提取物 (EE)、氯仿 (CF) 和甲醇 (MF) 馏分的镇痛活性。使用 S. cordifolia 的叶子来生产粗乙醇提取物，然后进行 CF 和 MF。在使用谷氨酸和福尔马林诱导的口腔痛觉过敏的瑞士小鼠中进行了实验。在福尔马林试验中，EE、CF 和 MF 的所有剂量均显著减少了第一期（p < 0.001）和第二期（p < 0.001）的口腔痛觉过敏，这也是纳洛酮敏感的。在谷氨酸诱导的痛觉过敏试验中，只有 CF 和 MF 显著减少了口腔痛觉过敏行为，抑制百分率分别为 48.1%（100mg/kg，CF）、56.1%（200mg/kg，CF）、66.4%（400mg/kg，CF）、48.2（200mg/kg，MF）和 60.1（400mg/kg，MF）。此外，用 EE、CF 和 MF 治疗动物不能引起运动活动变化。这些数据表明，S. cordifolia 对口腔痛觉过敏具有明显的镇痛活性。然而，有必要进行药理学和化学研究，以表征这种镇痛作用的负责机制，并鉴定 S. cordifolia 中存在的其他生物活性化合物。
