Departamento de Fisiologia, Universidade Federal de Sergipe (UFS), Campus Universitário, 49100-000, São Cristóvão-SE, Brazil.
J Ethnopharmacol. 2013 Mar 7;146(1):192-7. doi: 10.1016/j.jep.2012.12.031. Epub 2012 Dec 28.
Hyptis fruticosa is a plant native to Brazil with antinociceptive and antiinflamatory properties. This study evaluated the antinociceptive activity of the hydroethanol extract of the plant leaves (CHEE) against orofacial pain as well as its in vitro effect against lipid peroxidation.
The antinociceptive activity was investigated in mice orally treated with different doses of the CHEE (50, 100, and 200 mg/kg) and morphine (5 mg/kg) using formalin, glutamate, and capsaicin orofacial pain models using. Lipoperoxidation was induced in egg yolk by AAPH and FeSO4 in the absence and presence of the CHEE (5, 50, 100, and 150 μg/mL).
CHEE (200 mg/kg) significantly reduced (ρ<0.001) the pain response in the first (69.6%) and second (81.8%) phases of the formalin test, while the nociception caused by capsaicin was significantly (ρ<0.001) reduced by up to 62% at 200 mg/kg of extract. When glutamate was used as algogen, a significant (ρ<0.001) nociception reduction of up to 85% at 200 mg/kg extract was observed. CHEE showed a higher protection against lipoperoxidation caused by FeSO4 (82.3% TBARS inhibition) than AAPH (35.7% TBARS inhibition) at 150 μg/mL.
Hyptis fruticosa leaf CHEE is of pharmacological interest because it was able to inhibit the peripheral and central transmission of orofacial pain, while reducing the spreading of the inflammatory processes by neutralizing reactive oxygen species, which are by-products in the biosynthesis of pain mediators.
Hyptis fruticosa 是一种原产于巴西的植物，具有镇痛和抗炎特性。本研究评估了植物叶片的水乙醇提取物 (CHEE) 对口腔疼痛的镇痛活性及其对脂质过氧化的体外作用。
使用甲醛、谷氨酸和辣椒素口腔疼痛模型，通过口服给予不同剂量的 CHEE（50、100 和 200mg/kg）和吗啡（5mg/kg），研究了镇痛活性。在卵黄中通过 AAPH 和 FeSO4 在不存在和存在 CHEE（5、50、100 和 150μg/mL）的情况下诱导脂质过氧化。
CHEE（200mg/kg）显著降低（ρ<0.001）甲醛测试第一（69.6%）和第二（81.8%）阶段的疼痛反应，而辣椒素引起的疼痛反应在 200mg/kg 提取物时被显著（ρ<0.001）降低了 62%。当谷氨酸用作致痛剂时，观察到提取物 200mg/kg 时疼痛反应降低高达 85%。CHEE 在 150μg/mL 时对 FeSO4 引起的脂质过氧化（TBARS 抑制 82.3%）的保护作用高于 AAPH（TBARS 抑制 35.7%）。
Hyptis fruticosa 叶 CHEE 具有药理学意义，因为它能够抑制口腔疼痛的外周和中枢传递，同时通过中和活性氧来减轻炎症过程的扩散，活性氧是疼痛介质生物合成的副产物。
