Jiang C D, Li S, Deng C Y
School of Life Science, Southwest University, Chongqing 400715, China.
Genetika. 2011 Apr;47(4):537-42.
Imprinted genes play significant roles in the regulation of fetal growth and development, function of the placenta, and maternal nurturing behaviour in mammals. At present, few imprinted genes have been reported in pigs compared to human and mouse. In order to increase understanding of imprinted genes in swine, a polymorphism-based approach was used to assess the imprinting status of three porcine genes in 12 tissue types, obtained from F1 pigs of reciprocal crosses between Rongchang and Landrace pure breeds. In contrast to human and mouse homologues, porcine PPP1R9A was not imprinted, and was found to be expressed in all tissues examined. The expression of porcine NAP1L5 was detected in pituitary, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, ovary, and uterus, but undetectable in heart. Furthermore, porcine NAP1L5 was paternally expressed in the tissues where it's expression was observed. For PEG3, pigs expressed the paternal allele in skeletal muscle, liver, spleen, kidney, and uterus, but biallele in heart, lung, fat, stomach, small intestine, and ovary. Our data indicate that tissue distribution of the three gene differs among mammals, and the imprinting of NAP1L5 and PEG3 is well conserved.
印记基因在哺乳动物胎儿生长发育、胎盘功能以及母体养育行为的调控中发挥着重要作用。目前，与人类和小鼠相比，猪中报道的印记基因较少。为了增进对猪中印记基因的了解，采用了基于多态性的方法来评估荣昌猪和长白猪纯系正反交F1代猪的12种组织类型中三个猪基因的印记状态。与人类和小鼠同源基因不同，猪PPP1R9A没有印记，且在所有检测组织中均有表达。猪NAP1L5在垂体、肝脏、脾脏、肺、肾脏、胃、小肠、骨骼肌、脂肪、卵巢和子宫中均有表达，但在心脏中未检测到。此外，猪NAP1L5在所观察到其表达的组织中呈父本表达。对于PEG3，猪在骨骼肌、肝脏、脾脏、肾脏和子宫中表达父本等位基因，但在心脏、肺、脂肪、胃、小肠和卵巢中为双等位基因表达。我们的数据表明，这三个基因在不同哺乳动物中的组织分布存在差异，并且NAP1L5和PEG3的印记具有良好的保守性。
