猪胎盘 DCN、PON2 和 PEG3 基因的表达及基因组印迹

Expression and genomic imprinting of DCN, PON2 and PEG3 genes in porcine placenta.

机构信息

Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, PR China.

出版信息

Anim Reprod Sci. 2011 Jan;123(1-2):70-4. doi: 10.1016/j.anireprosci.2010.11.012. Epub 2010 Nov 30.

DOI:10.1016/j.anireprosci.2010.11.012

PMID:21232885

Abstract

Imprinted genes play vital roles in the placental development and fetal growth in eutherian mammals. DCN (decorin), PON2 (paraoxonase 2) and PEG3 (paternally expressed 3) genes have been identified as imprinted genes in the mouse. Here, we detected the imprinting status of three genes in the porcine placenta on DG90 (day 90 of gestation) and the expression differences in Yorkshire and Meishan placenta on DG26, DG55 and DG90. The results indicated that the DCN and PON2 genes were not imprinted genes, while the PEG3 gene showed paternal monoallelic expression in porcine placenta. The expression of the DCN gene increased from DG26 to DG90 in both Yorkshire and Meishan pig placenta. However, this gene expression was greater in Yorkshire than Meishan pig on DG55. The expression of the PON2 gene was greater in Meishan pig than that in Yorkshire on DG26 and DG90. The PEG3 gene expression was not affected by day of pregnancy or breed. Data from the present study contribute to function genomic of porcine placental development.

摘要

印迹基因在真兽类哺乳动物的胎盘发育和胎儿生长中起着至关重要的作用。DCN（decorin）、PON2（对氧磷酶 2）和 PEG3（父源表达 3）基因已被确定为小鼠中的印迹基因。在这里，我们检测了 DG90 （妊娠第 90 天）猪胎盘中三个基因的印迹状态以及 DG26、DG55 和 DG90 时 Yorkshire 和 Meishan 胎盘中的表达差异。结果表明，DCN 和 PON2 基因不是印迹基因，而 PEG3 基因在猪胎盘中表现出父系单等位基因表达。DCN 基因的表达在 Yorkshire 和 Meishan 猪胎盘中均从 DG26 增加到 DG90。然而，该基因在 DG55 时在 Yorkshire 中的表达大于 Meishan。PON2 基因的表达在 DG26 和 DG90 时在 Meishan 猪中大于 Yorkshire。PEG3 基因的表达不受妊娠天数或品种的影响。本研究的数据有助于猪胎盘发育的功能基因组学研究。