人副流感病毒2型融合蛋白基因及其3'基因间隔区的序列：缺乏小疏水（SH）基因。

Sequence of the fusion protein gene of human parainfluenza type 2 virus and its 3' intergenic region: lack of small hydrophobic (SH) gene.

作者信息

Kawano M, Bando H, Ohgimoto S, Kondo K, Tsurudome M, Nishio M, Ito Y

机构信息

Department of Microbiology, Mie University School of Medicine, Tokyo, Japan.

出版信息

Virology. 1990 Sep;178(1):289-92. doi: 10.1016/0042-6822(90)90406-h.

DOI:10.1016/0042-6822(90)90406-h

PMID:2167555

Abstract

cDNA clones representing the fusion (F) gene of human parainfluenza virus type 2 (PIV-2) were isolated from cDNA libraries constructed from virus-specific mRNA and genomic RNA, and the complete nucleotide sequence of the F gene was determined. The F gene is 1854 nucleotides long and encodes one long open reading frame of 551 amino acids. The cleavage site for activation of the precursor Fo protein is Thr-Arg-Gln-Lys-Arg. The F gene of PIV-2 is most closely related to those of simian virus 5 (SV5) and mumps virus (MuV). Interestingly, although the HN glycoprotein of PIV-2 shows no relatedness to the HA glycoprotein of measles virus (MV), a distinct homology is found in the F proteins of PIV-2 and MV. As concerns F proteins, paramyxoviruses can be divided into two subgroups; that is, PIV-2, SV5, and MuV belong to one group, and HPIV-1, SV, and PIV-3 belong to the other group. Newcastle disease virus (NDV) and MV are intermediate. Coding regions for small hydrophobic (SH) proteins have been found between the HN and F genes of SV5 and MuV, which are the viruses most closely related to PIV-2. However, such a gene could not be detected in two different strains of PIV-2.

摘要

从由病毒特异性mRNA和基因组RNA构建的cDNA文库中分离出代表2型人副流感病毒（PIV - 2）融合（F）基因的cDNA克隆，并确定了F基因的完整核苷酸序列。F基因长1854个核苷酸，编码一个由551个氨基酸组成的长开放阅读框。前体Fo蛋白激活的切割位点是苏氨酸 - 精氨酸 - 谷氨酰胺 - 赖氨酸 - 精氨酸。PIV - 2的F基因与猴病毒5（SV5）和腮腺炎病毒（MuV）的F基因关系最为密切。有趣的是，尽管PIV - 2的HN糖蛋白与麻疹病毒（MV）的HA糖蛋白没有相关性，但在PIV - 2和MV的F蛋白中发现了明显的同源性。关于F蛋白，副粘病毒可分为两个亚组；也就是说，PIV - 2、SV5和MuV属于一组，HPIV - 1、SV和PIV - 3属于另一组。新城疫病毒（NDV）和MV处于中间位置。在与PIV - 2关系最密切的病毒SV5和MuV的HN和F基因之间发现了小疏水（SH）蛋白的编码区。然而，在两种不同的PIV - 2毒株中未检测到这样的基因。