Chen G M, Hung T, Mackow E R
Department of Microbiology and Immunology, Stanford University, California 94305.
Virology. 1990 Sep;178(1):311-5. doi: 10.1016/0042-6822(90)90411-j.
Gene segment 9 of the adult diarrheal rotavirus, ADRV, has been cloned, and the full-length gene 9 cDNA has been sequenced. Sequences at the 5' and 3' termini of the gene 9 double-stranded RNA were confirmed by direct RNA sequencing. The ninth RNA segment is 814 bases in length with a single open reading frame beginning at base 10 and terminating at base 757. The encoded polypeptide contains 249 amino acids with a calculated molecular weight of 28.5 kDa. The protein contains three potential N-linked glycosylation sites and a hydrophobic signal-like sequence at its amino terminus. A search of the NBRF protein data base with the encoded polypeptide revealed extensive similarities with VP7 proteins from a number of group A rotaviruses. Direct comparisons of the ADRV gene 9 polypeptide and the group A rotavirus VP7 demonstrate that the two proteins share 78% amino acid similarity and 28% identity as well as predicted secondary structure similarities. These findings suggest that the ADRV gene segment 9 encodes the VP7 polypeptide equivalent of group A rotaviruses.
成人腹泻轮状病毒（ADRV）的基因片段9已被克隆，并且全长基因9的cDNA已被测序。通过直接RNA测序确认了基因9双链RNA 5'和3'末端的序列。第九个RNA片段长度为814个碱基，有一个单一的开放阅读框，从第10个碱基开始，到第757个碱基结束。编码的多肽含有249个氨基酸，计算分子量为28.5 kDa。该蛋白质在其氨基末端含有三个潜在的N-连接糖基化位点和一个疏水信号样序列。用编码的多肽搜索NBRF蛋白质数据库发现与许多A组轮状病毒的VP7蛋白有广泛的相似性。ADRV基因9多肽与A组轮状病毒VP7的直接比较表明，这两种蛋白质具有78%的氨基酸相似性和28%的同一性以及预测的二级结构相似性。这些发现表明，ADRV基因片段9编码A组轮状病毒的VP7多肽等同物。
