Mackow E R, Fay M E, Shaw R, Tao H, Chen G
Northport VA Medical Center, New York 11768.
Virology. 1994 May 15;201(1):162-8. doi: 10.1006/viro.1994.1279.
Adult diarrheal rotavirus, ADRV, is a noncultivable human group B rotavirus. A complete cDNA copy of ADRV gene segment 2 has been cloned and sequenced. Gene segment 2 contains 2844 bases and encodes one long open reading frame beginning at base 14 and terminating at base 2812. Gene 2 encodes a protein containing 933 amino acids with a calculated molecular weight of 105.6 kDa and a pl of 5.5. The gene 2 polypeptide contains significant homology with the VP2 protein which comprises the core of group A rotavirus strains. The gene 2 protein has been expressed in a rabbit reticulocyte lysate in vitro and is identical in molecular mass with a protein previously demonstrated to be present on iodinated EDTA-treated virions and from in vitro translations of total ADRV mRNA. A recombinant baculovirus containing gene segment 2 has been constructed and used to express the encoded VP2 equivalent protein. The expressed VP2 protein is immunoprecipitable by hyperimmune anti-ADRV serum, porcine group B infection serum, and human convalescent serum but not by hyperimmune serum to group A rotavirus. Our results suggest that ADRV gene segment 2 encodes the VP2 protein equivalent to group A rotavirus strains present in the core of the group B virion.
成人腹泻轮状病毒（ADRV）是一种不可培养的人类B组轮状病毒。已克隆并测序了ADRV基因片段2的完整cDNA拷贝。基因片段2含有2844个碱基，编码一个从第14个碱基开始至第2812个碱基终止的长开放阅读框。基因2编码一种含有933个氨基酸的蛋白质，计算分子量为105.6 kDa，等电点为5.5。基因2多肽与构成A组轮状病毒株核心的VP2蛋白具有显著同源性。基因2蛋白已在兔网织红细胞裂解物中体外表达，其分子量与先前证明存在于碘化EDTA处理的病毒体上以及ADRV总mRNA体外翻译产物中的一种蛋白质相同。构建了一个含有基因片段2的重组杆状病毒，并用于表达编码的VP2等效蛋白。表达的VP2蛋白可被超免疫抗ADRV血清、猪B组感染血清和人恢复期血清免疫沉淀，但不能被A组轮状病毒超免疫血清免疫沉淀。我们的结果表明，ADRV基因片段2编码的VP2蛋白等同于存在于B组病毒体核心中的A组轮状病毒株。
