Lautier R, Achtelik W V, Wolff H H
Klinik für Dermatologie und Venerologie, Medizinischen Universität zu Lübeck.
Z Hautkr. 1990 Jun;65(6):571-4.
Using the PAP technique, paraffin-embedded and formalin-fixed tissue sections taken from 11 patients suffering from Paget's disease (5 mammary, 6 extramammary cases) were stained with antibodies against keratin, actin, S 100 protein, CEA, HMFG 1, cytokeratin 6 and 18 (Dako-CK-1), as well as the lectin SBA. We did not find any differences between mammary and extramammary Paget's disease. In both forms of the disease, all tumor cells showed reaction to antibodies against HMFG 1 and CEA and, in 50 to 100%, to the lectin SBA. The other antibodies investigated did not react with Paget's cells. Since HMFG 1 and Dako-CK-1 are selective markers of apocrine and eccrine sweat gland structures, respectively, our results suggest apocrine differentiation of both mammary and extramammary Paget's disease. At the same time, our findings rule out a possible histogenesis of Paget's cells from melanocytes (positive for S 100 protein) or keratinocytes (positive for Dako-CK-1 and antikeratin).
采用PAP技术，对11例佩吉特病患者（5例乳腺病例，6例乳腺外病例）的石蜡包埋、福尔马林固定组织切片，用抗角蛋白、肌动蛋白、S100蛋白、癌胚抗原（CEA）、人乳脂肪球膜抗原1（HMFG 1）、细胞角蛋白6和18（Dako - CK - 1）抗体以及凝集素大豆凝集素（SBA）进行染色。我们未发现乳腺佩吉特病与乳腺外佩吉特病之间存在任何差异。在这两种疾病形式中，所有肿瘤细胞均对针对HMFG 1和CEA的抗体呈反应，且50%至100%的肿瘤细胞对凝集素SBA呈反应。所研究的其他抗体均未与佩吉特细胞发生反应。由于HMFG 1和Dako - CK - 1分别是顶泌汗腺和小汗腺结构的选择性标志物，我们的结果提示乳腺和乳腺外佩吉特病均有顶泌汗腺分化。同时，我们的研究结果排除了佩吉特细胞可能来源于黑素细胞（S100蛋白阳性）或角质形成细胞（Dako - CK - 1和抗角蛋白阳性）的组织发生情况。
