Bacteriology Unit, National Institute for Health and Welfare, Helsinki, Finland.
Foodborne Pathog Dis. 2011 Oct;8(10):1143-5. doi: 10.1089/fpd.2011.0882. Epub 2011 Jun 15.
The genetic diversity of Campylobacter jejuni strains isolated from Finnish patients was studied by typing 508 strains by pulsed-field gel electrophoresis: 311 were isolated from domestically acquired infections and 197 from travel-related infections. The strains were systematically selected from a larger collection of previously serotyped strains isolated during two 1-year sampling periods. The four most common SmaI profiles accounted for 45% of the domestic strains but only 3% of the travel-related strains. Of the domestic strains, 69% belonged to SmaI subtypes found during both sampling periods. The predominating SmaI subtypes and strains that were not digested by SmaI were typed by KpnI. Analyzing the temporal diversity of the pulsed-field gel electrophoresis profiles revealed six common persistent SmaI/KpnI subtypes among the domestic strains. Five of them have been identified in cattle, and two in chickens with a temporal association with human infections.
本研究通过脉冲场凝胶电泳对 508 株分离自芬兰患者的空肠弯曲菌菌株进行基因多样性分析：311 株为本地感染菌株，197 株为旅行相关感染菌株。这些菌株是从先前在两个为期一年的采样期间分离的、经血清学分型的更大菌株集合中系统选择的。4 种最常见的 SmaI 图谱占国内菌株的 45%，但仅占旅行相关菌株的 3%。在国内菌株中，69%属于在两个采样期间都发现的 SmaI 亚型。SmaI 未消化的菌株和主要 SmaI 亚型用 KpnI 进行了分型。分析脉冲场凝胶电泳图谱的时间多样性，发现本地菌株中存在 6 种常见的持续性 SmaI/KpnI 亚型。其中 5 种在牛中被发现，2 种在鸡中被发现，与人类感染有关。
