Imai Y, Kikuchi M, Matsuda M, Honda M, Fukuyama M, Tsukada M, Kaneuchi C
Department of Biosciences, College of Environmental Health, Azabu University, Sagamihara, Japan.
Cytobios. 1994;78(313):115-22.
Chromosomal genomic DNA from 25 strains of thermophilic Campylobacter coli and C. jejuni were analysed after pulsed-field gel electrophoresis (PFGE) of restriction fragments generated by SalI and SmaI to evaluate the utility of macro-fingerprinting analysis and to assess the possible subtyping of the isolates. Two rare-cutting restriction enzymes, SalI and SmaI, allowed the subclassification of eight strains of C. coli into six types. SalI facilitated the subclassification of seventeen strains of C. jejuni into thirteen types and SmaI the subclassification of seventeen strains into twelve types. The diversity in the PFGE profiles was demonstrated and, therefore, the two enzymes were found to be potentially useful for detecting variability of PFGE profiles among strains of C. coli and C. jejuni. The two enzymes and PFGE may be useful in molecular epidemiology.
对来自25株嗜热空肠弯曲菌和结肠弯曲菌的染色体基因组DNA进行分析，在对由SalI和SmaI产生的限制性片段进行脉冲场凝胶电泳（PFGE）之后，评估宏观指纹分析的效用并评估分离株可能的亚型分类。两种稀有切割限制性酶SalI和SmaI可将8株结肠弯曲菌细分为6种类型。SalI可将17株空肠弯曲菌细分为13种类型，SmaI可将17株细分为12种类型。PFGE图谱中的多样性得到了证明，因此，发现这两种酶对于检测结肠弯曲菌和空肠弯曲菌菌株之间PFGE图谱的变异性可能有用。这两种酶和PFGE可能在分子流行病学中有用。
