汉方药对肿瘤启动子诱导的爱泼斯坦-巴尔病毒抗原的抑制作用。

Inhibitory effects of kampo medicine on Epstein-Barr virus antigen induction by tumor promoter.

作者信息

Furukawa M, Sakashita H, Kamide M, Umeda R

机构信息

Department of Otorhinolaryngology, School of Medicine, Kanazawa University, Japan.

出版信息

Auris Nasus Larynx. 1990;17(1):49-54. doi: 10.1016/s0385-8146(12)80021-6.

DOI:10.1016/s0385-8146(12)80021-6

PMID:2167654

Abstract

We have investigated the possible interaction of Japanese Kampo medicine; Shosaiko-to with Epstein-Barr virus (EBV) induction by TPA (12-O-tetra-decanoyl-phorbol-13-acetate) in EBV-genome harboring cells. The effect of ether extract of Shosaiko-to was also studied on the induction of EBV by superinfection with EBV from P3HR-1 cells (P3H-EBV). Concomitant treatment of EBV-latently-infected cells (Raji and A2L/AH) with TPA and ether extract resulted in effective inhibition of EBV-antigen induction. However, in the experiments to test the influence of ether extract on EBV induction by superinfecting Raji cells with P3H-EBV, ether extract did not affect the percentage of EBV-antigen-positive cells. These data indicate that Shosaiko-to is effective to inhibit antigen synthesis induced by chemical promoters without affecting EBV-antigen induction after superinfection.

摘要

我们研究了日本汉方药物小柴胡汤与12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）在携带EB病毒基因组的细胞中诱导EB病毒（EBV）之间可能存在的相互作用。还研究了小柴胡汤乙醚提取物对P3HR - 1细胞来源的EB病毒（P3H - EBV）超感染诱导EBV的影响。用TPA和乙醚提取物同时处理EB病毒潜伏感染细胞（Raji和A2L/AH）可有效抑制EB病毒抗原的诱导。然而，在测试乙醚提取物对P3H - EBV超感染Raji细胞诱导EBV影响的实验中，乙醚提取物并未影响EB病毒抗原阳性细胞的百分比。这些数据表明，小柴胡汤可有效抑制化学启动子诱导的抗原合成，而不影响超感染后EB病毒抗原的诱导。