Center for Biostabilization, University of California, Davis, California 95616.
Integr Comp Biol. 2005 Nov;45(5):810-20. doi: 10.1093/icb/45.5.810.
The Center for Biostabilization at UC Davis is attempting to stabilize mammalian cells in the dry state. We review here some of the lessons from nature that we have been applying to this enterprise, including the use of trehalose, a disaccharide found at high concentrations in many anhydrobiotic organisms, to stabilize biological structures, both in vitro and in vivo. Trehalose has useful properties for this purpose and in at least in one case-human blood platelets-introducing this sugar may be sufficient to achieve useful stabilization. Nucleated cells, however, are stabilized by trehalose only during the initial stages of dehydration. Introduction of a stress protein obtained from an anhydrobiotic organism, Artemia, improves the stability markedly, both during the dehydration event and following rehydration. Thus, it appears that the stabilization will require multiple adaptations, many of which we propose to apply from studies on anhydrobiosis.
加州大学戴维斯分校的生物稳定中心（Center for Biostabilization at UC Davis）正试图使哺乳动物细胞在干燥状态下稳定化。我们在此回顾了一些从自然界中获得的经验，这些经验已被应用于这一领域，包括使用海藻糖（trehalose），一种在许多耐旱生物中高浓度存在的二糖，以稳定生物结构，无论是在体外还是体内。海藻糖具有这种用途的有用特性，并且在至少一种情况下——人类血小板——引入这种糖可能足以实现有用的稳定化。然而，有核细胞仅在脱水的初始阶段通过海藻糖稳定化。从耐旱生物卤虫（Artemia）中获得的应激蛋白的引入，显著提高了稳定性，无论是在脱水过程中还是在再水合之后。因此，似乎需要多种适应来实现稳定化，我们建议从对耐旱生物的研究中应用其中许多适应。
