Toshimori K, Araki S, Oura C
Department of Anatomy, Miyazaki Medical College, Japan.
Arch Histol Cytol. 1990 Jul;53(3):339-49. doi: 10.1679/aohc.53.339.
A monoclonal antibody T21 specifically recognizes the mouse epididymal sialoglycoprotein of 54,000 dalton (SGP54). The localization of SGP54 was studied in the epididymal duct of germ cell-free WBB6F1W/Wv mutant mice (W/Wv mice) by avidin biotin complex (ABC) immunohistochemistry using T21. None of the testis cells showed immunoreaction. No spermatozoa were present in the epididymal duct lumen. The duct luminal fluid was stained weakly in the proximal corpus epididymidis, and strongly in the cauda epididymidis. Degenerated cells appeared in the duct lumen. The degenerated cells located at the corpus epididymidis showed strong immunostaining in the cytoplasmic region, while the degenerated cells located at the cauda epididymidis showed weak immunostaining. Immunoreaction was also detected between and on microvilli along the epididymis, the intensity being very strong at the distal caput and proximal corpus epididymidis. Invaginations and coated vesicles at the luminal surface of the principal cells were frequently immunostained at the corpus epididymidis. Giant inclusions frequently occurred in the principal cells of the distal caput and corpus epididymidis, with these being very intensely immunostained. These inclusions are ultrastructurally confirmed to be giant multivesicular bodies reported by ABE et al. (1984) in the mouse with the efferent duct cutting. These results suggest that the majority of excess SGP54 are absorbed by the principal cells at the distal caput to corpus epididymidis and catalyzed in the giant multivesicular bodies.
单克隆抗体T21能特异性识别54000道尔顿的小鼠附睾唾液糖蛋白（SGP54）。利用T21，通过抗生物素蛋白-生物素复合物（ABC）免疫组织化学方法，研究了无生殖细胞的WBB6F1W/Wv突变小鼠（W/Wv小鼠）附睾管中SGP54的定位。睾丸细胞均未显示免疫反应。附睾管腔中没有精子。附睾管腔液在附睾体近端染色较弱，在附睾尾染色较强。管腔中出现了退化细胞。位于附睾体的退化细胞在细胞质区域显示强免疫染色，而位于附睾尾的退化细胞显示弱免疫染色。在附睾沿微绒毛之间及微绒毛上也检测到免疫反应，在附睾头远端和附睾体近端强度非常强。主细胞腔表面的内陷和被膜小泡在附睾体经常被免疫染色。巨大包涵体经常出现在附睾头远端和附睾体的主细胞中，这些包涵体被非常强烈地免疫染色。这些包涵体在超微结构上被证实是ABE等人（1984年）在切断输出小管的小鼠中报道的巨大多囊泡体。这些结果表明，大部分多余的SGP54被附睾头远端到附睾体的主细胞吸收，并在巨大多囊泡体中被催化。
