Martín R, Santamaría L, Fraile B, Paniagua R, Polak J M
Department of Morphology (Histology), School of Medicine, Autonomous University, Madrid, Spain.
Histochem J. 1995 Jun;27(6):431-9.
The distribution of protein gene product 9.5 (PGP) and ubiquitin in the spermatozoa and epithelial cells in the different regions of the rat ductus epididymidis (proximal caput, distal caput, corpus and cauda) was studied by Western blotting analyses and electron microscopical immunogold labelling. Western blotting analyses showed that the PGP immunoreactive band was very intense in the caput and cauda epididymidis and almost irrelevant in the corpus, while the ubiquitin immunoreactive band was intense in the distal caput and cauda. No ubiquitin immunoreactive band was observed in the proximal caput and only a very weak band was seen in the corpus. The results of electron microscopical immunogold labelling varied from one epididymal region to another. The proximal caput epididymidis presented immunoreaction to PGP in the rough endoplasmic reticulum, cytosol, mitochondria and microvilli of most principal cells, and in the cytosol, rough endoplasmic reticulum and mitochondria of most basal cells. No ubiquitin immunoreaction was observed in this epididymal region. In the distal caput epididymidis, PGP immunoreactivity was detected in some principal and basal cells in the same intracellular locations as described in the proximal caput. In this region, ubiquitin immunoreactivity appears in the apical cytosol and mitochondria of principal cells. The corpus epididymidis showed no immunoreaction to PGP or ubiquitin. In the cauda epididymidis, immunostaining to PGP was observed in most clear cells and in isolated principal cells. The intracellular location of PGP in both cell types was the cytosol, mitochondria and microvilli. Ubiquitin immunoreactivity was detected in the perinuclear cytosol and mitochondria-but not in the digestive vacuoles-of some clear cells.(ABSTRACT TRUNCATED AT 250 WORDS)
通过蛋白质印迹分析和电子显微镜免疫金标记法，研究了大鼠附睾管不同区域（近端头部、远端头部、体部和尾部）精子和上皮细胞中蛋白质基因产物9.5（PGP）和泛素的分布。蛋白质印迹分析表明，PGP免疫反应条带在附睾头部和尾部非常强烈，在体部几乎没有，而泛素免疫反应条带在远端头部和尾部强烈。在近端头部未观察到泛素免疫反应条带，在体部仅可见非常弱的条带。电子显微镜免疫金标记的结果因附睾区域而异。近端附睾头部的大多数主细胞的粗面内质网、细胞质、线粒体和微绒毛，以及大多数基底细胞的细胞质、粗面内质网和线粒体中呈现PGP免疫反应。在该附睾区域未观察到泛素免疫反应。在远端附睾头部，在一些主细胞和基底细胞的相同细胞内位置检测到PGP免疫反应性，与近端头部所述相同。在该区域，泛素免疫反应出现在主细胞的顶端细胞质和线粒体中。附睾体部对PGP或泛素均无免疫反应。在附睾尾部，在大多数透明细胞和孤立的主细胞中观察到PGP免疫染色。两种细胞类型中PGP的细胞内位置是细胞质、线粒体和微绒毛。在一些透明细胞的核周细胞质和线粒体中检测到泛素免疫反应性，但在消化泡中未检测到。（摘要截短于250字）
