Abe K, Takano H, Ito T
Arch Histol Jpn. 1984 Jun;47(2):137-47. doi: 10.1679/aohc.47.137.
The efferent duct or the epididymal duct at the border between the caput and corpus epididymidis was cut or ligated in mice at the following stages: birth, 20 days, and 60 days of age. The epididymal duct of the corpus epididymidis was observed at 60 days of age after neonatal or juvenile operation and 4 weeks after the adult operation. After efferent duct interruption, the epididymal duct in the corpus possessed abundant PAS-positive material with no spermatozoa in the lumen, and the principal cells of the epithelium contained PAS-positive inclusions which were considered to be formed due to absorption of the PAS-positive material secreted by the principal cells in the caput epididymidis. Especially after interruption at the border between the caput and corpus epididymidis in juvenile mice, the epithelium of the epididymal duct in the corpus was much taller with peculiar pale, vacuolated cells. These cells were considered as abnormally differentiated forms of the principal cells. The occurrence of the PAS-positive inclusions and peculiar pale cells varied in frequency according to the level and age of interruption of the epididymal duct. The results suggest that the luminal contents produced in the proximal region of the duct may exert an influence on the differentiation of the principal cells of the epididymal duct.
在出生、20日龄和60日龄的小鼠中，分别在附睾头与附睾体交界处切断或结扎输出小管或附睾管。在新生或幼年手术后60日龄以及成年手术后4周，观察附睾体的附睾管。输出小管中断后，附睾体的附睾管腔内有丰富的PAS阳性物质且无精子，上皮主细胞含有PAS阳性包涵体，这些包涵体被认为是由于吸收了附睾头主细胞分泌的PAS阳性物质而形成的。特别是幼年小鼠在附睾头与附睾体交界处中断后，附睾体附睾管的上皮细胞高得多，有特殊的浅色空泡状细胞。这些细胞被认为是主细胞的异常分化形式。PAS阳性包涵体和特殊浅色细胞的出现频率根据附睾管中断的水平和年龄而有所不同。结果表明，附睾管近端区域产生的管腔内容物可能对附睾管主细胞的分化产生影响。
