Walter and Eliza Hall Institute of Medical Research, Parkville, Melbourne, Victoria 3052, Australia.
J Immunol. 2011 Jul 15;187(2):842-50. doi: 10.4049/jimmunol.1101176. Epub 2011 Jun 15.
Three surface molecules of mouse CD8(+) dendritic cells (DCs), also found on the equivalent human DC subpopulation, were compared as targets for Ab-mediated delivery of Ags, a developing strategy for vaccination. For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants. For Ab production, however, Clec9A excelled as a target, even in the absence of adjuvant. Potent humoral immunity was a result of the highly specific expression of Clec9A on DCs, which allowed longer residence of targeting Abs in the bloodstream, prolonged DC Ag presentation, and extended CD4 T cell proliferation, all of which drove highly efficient development of follicular helper T cells. Because Clec9A shows a similar expression pattern on human DCs, it has particular promise as a target for vaccines of human application.
三种小鼠 CD8(+)树突状细胞（DC）的表面分子，也存在于等效的人类 DC 亚群中，被比较作为 Ab 介导的 Ag 传递的靶点，这是一种新兴的疫苗接种策略。对于细胞毒性 T 细胞的产生，DEC-205 和 Clec9A 是有效的靶点，但只有在佐剂存在的情况下才有效，而对于 Ab 的产生，Clec9A 则是一个出色的靶点，即使没有佐剂也是如此。强烈的体液免疫是由于 Clec9A 在 DC 上的高度特异性表达所致，这使得靶向 Abs 在血液中的停留时间更长，延长了 DC 呈递 Ag 的时间，并延长了 CD4 T 细胞的增殖，所有这些都促进了滤泡辅助 T 细胞的高效发育。由于 Clec9A 在人类 DC 上表现出相似的表达模式，因此它作为人类应用疫苗的靶点具有特别的前景。
