Eisenhardt A, Scherag A, Jöckel K H, Reis H, Rübben H, Siffert W
Praxisklinik Urologie Rhein-Ruhr, Mülheim an der Ruhr, Germany. Andreas.Eisenhardt @ googlemail.com
Urol Int. 2011;87(1):80-6. doi: 10.1159/000325398. Epub 2011 Jun 11.
G proteins are ubiquitously expressed signal transduction proteins playing a key role in multiple signal transduction pathways. The Gαs subunit has been considered as an apoptosis factor. In this study the role of GNAS T393C genotypes of the GNAS gene encoding Gαs was analyzed for its influence on the development and progression of prostate cancer.
Genotyping of the GNAS T393C polymorphism in 196 prostate cancer patients and 200 healthy controls was performed by DNA extraction followed by PCR and restriction analysis.
We observed no evidence of effects related to GNAS T393C genotype as demonstrated by a comparison of the genotype distribution in prostate cancer patients and healthy controls, the genotype distribution dependent on grade of the primary diagnosis or data on clinical follow-up.
In conclusion, this study did not demonstrate an association between the GNAS T393C genotype and prostate cancer though such a relationship has been described for other cancer entities.
G蛋白是广泛表达的信号转导蛋白，在多种信号转导途径中起关键作用。Gαs亚基被认为是一种凋亡因子。在本研究中，分析了编码Gαs的GNAS基因的GNAS T393C基因型对前列腺癌发生发展的影响。
通过DNA提取，随后进行PCR和限制性分析，对196例前列腺癌患者和200例健康对照者的GNAS T393C多态性进行基因分型。
通过比较前列腺癌患者和健康对照者的基因型分布、取决于初次诊断分级的基因型分布或临床随访数据，我们未观察到与GNAS T393C基因型相关的影响证据。
总之，本研究未证明GNAS T393C基因型与前列腺癌之间存在关联，尽管其他癌症实体已描述了这种关系。
