Department of Clinical Pathology, Cleveland Clinic, OH 44195, USA.
Pathobiology. 2011;78(2):115-21. doi: 10.1159/000296015. Epub 2011 Jun 14.
Phosphorylation is one of the most important processes in cell signal transduction. Detection of phosphorylated proteins in cancer tissue is useful for prognosis and diagnosis, and it might be very helpful in monitoring treatment using targeted therapy. For these reasons, the in situ quantitative measurement and subcellular localization of phosphoproteins will likely be important. However, phosphoproteins are extremely labile, a likely explanation for inconsistent or contradictory reports. Thus, the development of new paradigms for tissue handling, immunostaining, and quality control are needed.
磷酸化是细胞信号转导中最重要的过程之一。在癌症组织中检测磷酸化蛋白质对于预后和诊断非常有用，并且可能对使用靶向治疗进行监测治疗非常有帮助。基于这些原因，磷酸化蛋白质的原位定量测量和亚细胞定位可能非常重要。然而，磷酸化蛋白质非常不稳定，这可能是导致不一致或矛盾报告的原因。因此，需要开发新的组织处理、免疫染色和质量控制范例。
