Dion M, Hamelin C
Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides (Québec), Canada.
Can J Microbiol. 1990 May;36(5):341-7.
The whole human cytomegalovirus strain AD169 genome was cloned into plasmid pAT153 in the form of 25 HindIII fragments. Double and triple digestions of the recombinant plasmids with restriction endonucleases BamHI, BglII, ClaI, DraI, EcoRI, EcoRV, HindIII, HpaI, KpnI, PaeR7, PstI, SphI and XbaI yielded a detailed restriction map of human cytomegalovirus DNA. Knowing the exact position of numerous restriction sites in the viral DNA molecule, we have been able to examine very closely the heterologous region between the long and the short segments of the human cytomegalovirus genome.
整个人巨细胞病毒AD169株基因组以25个HindIII片段的形式克隆到质粒pAT153中。用限制性内切酶BamHI、BglII、ClaI、DraI、EcoRI、EcoRV、HindIII、HpaI、KpnI、PaeR7、PstI、SphI和XbaI对重组质粒进行双酶切和三酶切，得到了人巨细胞病毒DNA的详细限制性图谱。由于知道病毒DNA分子中众多限制性位点的确切位置，我们得以非常仔细地研究人巨细胞病毒基因组长短片段之间的异源区域。
