人巨细胞病毒Ad-169株转化序列在临床分离株DNA中的保守性及图谱定位
Conservation and map location of human cytomegalovirus strain Ad-169 transforming sequences in the DNA of clinical isolates.
作者信息
Somogyi T, Colimon R, Bertrand C, Michelson S
出版信息
Microbiologica. 1987 Jan;10(1):125-31.
PMID:3033447
Abstract
All DNAs from human cytomegalovirus studied (57 fresh isolates, 3 reference strains) showed hybridization under stringent conditions to a plasmid containing strain Ad-169 transforming sequences. Analysis of hybridization patterns obtained with DNAs digested by three different enzymes allowed construction of a global map for this highly conserved area of the genome.
摘要
所有研究的人巨细胞病毒DNA（57株新鲜分离株，3株参考菌株）在严格条件下均与含有Ad-169株转化序列的质粒发生杂交。对用三种不同酶消化的DNA所获得的杂交模式进行分析，使得能够构建该基因组高度保守区域的全局图谱。
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