Randolph V B, Stollar V
Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
J Gen Virol. 1990 Aug;71 ( Pt 8):1845-50. doi: 10.1099/0022-1317-71-8-1845.
Cell-to-cell fusion of Aedes albopictus (mosquito) cells infected with dengue and St Louis encephalitis (SLE) flaviviruses was induced by exposure to low pH. The parameters of this low pH-induced fusion were examined. Syncytium formation was maximal in cultures 36 to 48 h post-infection and occurred when cultures were maintained at the acid pH for 15 min at 35 degrees C. The optimal pH range for fusion was 5.0 to 6.5 for dengue virus-infected cells and 5.0 to 5.5 for SLE virus-infected cells. Syncytia were not observed in vertebrate cells (Vero and BHK) under these conditions despite similar virus yields. Fusion was shown to be ATP-dependent and could be prevented by the addition of either polyclonal antiviral antibodies or monoclonal antibody to the envelope glycoprotein. The lysosomotropic amine ammonium chloride inhibited the replication of SLE virus in both mosquito and vertebrate cells, consistent with the idea that low pH-induced fusion is necessary for virus entry into both types.
暴露于低pH值可诱导感染登革热病毒和圣路易斯脑炎（SLE）黄病毒的白纹伊蚊（蚊子）细胞发生细胞间融合。对这种低pH值诱导融合的参数进行了研究。在感染后36至48小时的培养物中，多核巨细胞形成达到最大值，并且当培养物在35摄氏度的酸性pH值下维持15分钟时发生。登革热病毒感染细胞的融合最佳pH范围为5.0至6.5，SLE病毒感染细胞的融合最佳pH范围为5.0至5.5。尽管病毒产量相似，但在这些条件下，未在脊椎动物细胞（Vero和BHK）中观察到多核巨细胞。融合显示为ATP依赖性，并且可以通过添加多克隆抗病毒抗体或包膜糖蛋白单克隆抗体来阻止。溶酶体促渗胺氯化铵抑制SLE病毒在蚊子和脊椎动物细胞中的复制，这与低pH值诱导融合是病毒进入这两种细胞类型所必需的观点一致。
