Randolph V B, Hardy J L
Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720.
J Gen Virol. 1988 Sep;69 ( Pt 9):2199-207. doi: 10.1099/0022-1317-69-9-2199.
Viral mutants that appeared during long-term persistent infections of mosquito cell cultures (Aedes albopictus, A. dorsalis and Culex tarsalis) with St Louis encephalitis virus were characterized. Evidence was obtained for the presence of temperature-sensitive mutants in the A. dorsalis and C. tarsalis persistently infected cultures, and small plaque mutants were predominant in all cultures except one of two cell cultures of C. tarsalis. Virus from persistently infected A. albopictus cell cultures was growth-restricted in Vero and C. tarsalis cells. One of two persistently infected A. dorsalis cell cultures also produced viral mutants that were growth-restricted in C. tarsalis cells. Further, Western blots of persistently infected A. albopictus cell extracts showed an overproduction of capsid (C) and envelope (E) structural proteins and reduced production of an Mr 27K protein (p27) which was immunologically related to the E protein. In contrast, the production of E and C proteins in persistently infected C. tarsalis cultures was consistent with the amount of infectious virus present, whereas p27 was relatively overproduced. These observations suggest that the host cell has an important influence on both the types and relative quantities of viral mutants that accumulate during long-term persistent infections.
对感染圣路易斯脑炎病毒的蚊细胞培养物（白纹伊蚊、背点伊蚊和致倦库蚊）长期持续感染期间出现的病毒突变体进行了特征分析。在背点伊蚊和致倦库蚊持续感染的培养物中获得了温度敏感突变体存在的证据，除致倦库蚊的两种细胞培养物之一外，小蚀斑突变体在所有培养物中占主导地位。来自持续感染白纹伊蚊细胞培养物的病毒在非洲绿猴肾细胞和致倦库蚊细胞中生长受限。两个持续感染的背点伊蚊细胞培养物之一也产生了在致倦库蚊细胞中生长受限的病毒突变体。此外，持续感染白纹伊蚊细胞提取物的蛋白质免疫印迹显示衣壳（C）和包膜（E）结构蛋白过量产生，而与E蛋白免疫相关的27K蛋白（p27）产生减少。相比之下，持续感染致倦库蚊培养物中E和C蛋白的产生与存在的感染性病毒量一致，而p27相对过量产生。这些观察结果表明，宿主细胞对长期持续感染期间积累的病毒突变体的类型和相对数量都有重要影响。
