Busse E, Baum R P, Rohrbach E, Hör G, Kornhuber B
Abteilung für Hämatologie und Onkologie, Johann-Wolfgang-Goethe-Universität, Frankfurt/Main, BRD.
Nuklearmedizin. 1990 Jun;29(3):120-4.
The effect of triiodothyronine (T3) on the differentiation of cultured neuroblastoma (NB) cells was studied after 9 days of treatment with a dose of 10(-4) M/10(6) cells per day. Using phase contrast microscopy, 30-50% of NB cells showed formation of neurites as a morphological sign of cellular differentiation. The initial rise of the mitosis rate was followed by a plateau. Changes in cyclic nucleotide content, in the triphosphates and in the activity of the enzyme ornithine decarboxylase (ODC) were assessed in 2 human and 2 murine cell lines to serve as biochemical parameters of the cell differentiation induced by T3. Whereas the cAMP level increased significantly (3 to 7 fold compared with its initial value), the cGMP value dropped to 30 to 50% of that of the control group. ATP and GTP increased about 200%, the ODC showed a decrease of about 50%. The present studies show a biphasic effect of T3 on neuroblastoma cells: the initial rise of mitotic activity is followed by increased cell differentiation starting from day 4 of the treatment.
在用每天10(-4)M/10(6)个细胞的剂量处理9天后，研究了三碘甲状腺原氨酸（T3）对培养的神经母细胞瘤（NB）细胞分化的影响。使用相差显微镜观察，30%-50%的NB细胞显示出神经突形成，这是细胞分化的形态学标志。有丝分裂率最初上升，随后趋于平稳。在2个人类和2个鼠类细胞系中评估了环核苷酸含量、三磷酸含量以及鸟氨酸脱羧酶（ODC）活性的变化，作为T3诱导细胞分化的生化参数。虽然环磷酸腺苷（cAMP）水平显著升高（与其初始值相比增加了3至7倍），但环磷酸鸟苷（cGMP）值降至对照组的30%至50%。三磷酸腺苷（ATP）和三磷酸鸟苷（GTP）增加了约200%，ODC显示下降了约50%。目前的研究表明T3对神经母细胞瘤细胞有双相作用：有丝分裂活性最初上升，随后从处理第4天开始细胞分化增加。
