Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, People’s Republic of China.
J Virol. 2011 Aug;85(16):8427-30. doi: 10.1128/JVI.05016-11. Epub 2011 Jun 15.
Human norovirus (hNoV) remains refractory to propagation in cell culture systems. We believe that knowing the exact cell type that hNoV targets will provide important insights into culturing the virus. By the use of an in vitro whole-virus binding assay, the hNoV genogroup II genotype 4 Sakai variant was found to bind predominantly to cells of the lamina propria and Brunner's glands, but not to those of the luminal epithelial surface, of human duodenum tissue. Our findings, together with accumulating evidence reported elsewhere, suggest that hNoV may display tropism to nonepithelial cells, which is distinct from observations of other human enteric pathogens.
人诺如病毒（hNoV）仍然无法在细胞培养系统中繁殖。我们相信，了解 hNoV 靶向的确切细胞类型将为培养该病毒提供重要的见解。通过使用体外全病毒结合测定法，发现 hNoV 基因组 II 基因型 4 型佐贺变异株主要与人类十二指肠组织的固有层和 Brunner 腺细胞结合，但不与腔上皮表面的细胞结合。我们的研究结果与其他地方报道的累积证据一起表明，hNoV 可能表现出对非上皮细胞的嗜性，这与其他人类肠道病原体的观察结果不同。
