Division of Virology, Department of Microbiology and Immunology, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
J Virol. 2011 Aug;85(16):8449-52. doi: 10.1128/JVI.05054-11. Epub 2011 Jun 15.
Reassortment is important for influenza virus evolution and the generation of novel viruses with pandemic potential; however, the factors influencing reassortment are still poorly understood. Here, using reverse genetics and a replicon assay, we demonstrated that a mixed polymerase complex containing a pandemic (H1N1) 2009 influenza virus PB2 on a seasonal H1N1 virus background has reduced polymerase activity, leading to impaired virus viability. Adaptation of viruses containing the mixed polymerase complex resulted in compensatory mutations in PB1. Taken together, our results identify the cooperation between PB2 and PB1 as an important restricting factor for reassortment of influenza viruses.
重配对于流感病毒的进化和具有大流行潜力的新型病毒的产生非常重要；然而，影响重配的因素仍了解甚少。在这里，我们使用反向遗传学和复制子测定法，证明了含有大流行（H1N1）2009 流感病毒 PB2 的混合聚合酶复合物在季节性 H1N1 病毒背景下具有降低的聚合酶活性，导致病毒活力受损。含有混合聚合酶复合物的病毒的适应性导致 PB1 中的补偿性突变。总之，我们的结果确定了 PB2 和 PB1 之间的合作是流感病毒重配的一个重要限制因素。
