Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital, Huan-Hu-Xi-Road, He-Xi-District, Tianjin 300060, China.
Molecules. 2011 Jun 16;16(6):4958-70. doi: 10.3390/molecules16064958.
Gekko swinhonis Guenther has been used as an anti-cancer drug in traditional Chinese medicine for hundreds of years. Previous studies showed that the Gekko sulfated polysaccharide-protein complex suppressed the proliferation and migration of hepatoma cells. Gekko sulfated glycopeptide α was obtained from Gekko sulfated polysaccharide-protein complex using papain hydrolysis. Gekko sulfated glycopeptide α inhibited the proliferation and migration of SMMC-7721 cells. The secretion of IL-8 and the concentration of intracellular calcium were decreased after Gekko sulfated glycopeptide α exposure. SMMC-7721 cells in the control group showed abnormal features, with a polygonal shape, whereas this changed to a spindle shape after the treatment with Gekko sulfated glycopeptide α. Actin filaments were distributed diffusely along the cell membrane in control cells, whereas those were polymerized and preferentially accumulated in the cytoplasm of treated cells. Microtubules distributed in the cytoplasm of untreated cells were located diffusely whereas those in treated cells were polymerized. Therefore, Gekko sulfated glycopeptide α inhibit the migration of hepatoma cells via reducing the secretion of IL-8 and the concentration of intracellular calcium, as well as regulating the reorganization of cytoskeleton.
蛤蚧在传统中药中已被用作抗癌药物数百年。先前的研究表明，蛤蚧硫酸多糖-蛋白复合物抑制肝癌细胞的增殖和迁移。蛤蚧硫酸糖肽α是从蛤蚧硫酸多糖-蛋白复合物中用木瓜蛋白酶水解得到的。蛤蚧硫酸糖肽α抑制 SMMC-7721 细胞的增殖和迁移。暴露于蛤蚧硫酸糖肽α后，IL-8 的分泌和细胞内钙离子浓度降低。对照组的 SMMC-7721 细胞呈多边形，而经蛤蚧硫酸糖肽α处理后，细胞形态变为梭形。对照组细胞的肌动蛋白纤维沿细胞膜弥散分布，而处理组细胞的肌动蛋白纤维则在细胞质中聚合并优先聚集。未处理细胞的微管分布在细胞质中弥散，而处理细胞的微管则聚合。因此，蛤蚧硫酸糖肽α通过减少 IL-8 的分泌和细胞内钙离子浓度，以及调节细胞骨架的重排来抑制肝癌细胞的迁移。
