Nata K, Yonekura H, Yamamoto H, Okamoto H
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Biochem Biophys Res Commun. 1990 Aug 31;171(1):330-5. doi: 10.1016/0006-291x(90)91397-b.
By affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, we identified a novel cell surface receptor on intact rat cells, which bound, with similar dissociation constants, pancreatic polypeptide (PP), neuropeptide Y (NPY) and peptide YY (PYY), the members of the PP family. The receptor was detected on pancreatic islet and acinar cells, hepatocytes and epithelial cells of the stomach, duodenum and small intestine. Its molecular weight was estimated to be 65,000, and the cross-linking of [125I] labeled ligands was inhibited by an excess of unlabeled PP, NPY or PYY. The results suggest that the 65-kDa molecule is a common receptor for PP family peptides.
通过亲和交联和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，我们在完整的大鼠细胞上鉴定出一种新型细胞表面受体，该受体以相似的解离常数结合胰多肽（PP）、神经肽Y（NPY）和酪酪肽（PYY），它们均为PP家族成员。在胰岛、腺泡细胞、肝细胞以及胃、十二指肠和小肠的上皮细胞上均检测到该受体。其分子量估计为65,000，过量的未标记PP、NPY或PYY可抑制[125I]标记配体的交联。结果表明，65 kDa的分子是PP家族肽的共同受体。
