Lund T, Laland S G
Department of Biochemistry, University of Oslo, Norway.
Biochem Biophys Res Commun. 1990 Aug 31;171(1):342-7. doi: 10.1016/0006-291x(90)91399-d.
In vivo labelling of HeLa cells arrested in metaphase with [32P]-phosphate and in vitro phosphorylation of HMG I with the partially purified growth associated H1 kinase was used to study metaphase specific phosphorylation of HMG I. It was found that threonine 53 and 78 became phosphorylated. These amino acids are embedded in respectively the sequence PTPKR and TPGRK which are similar to the sequences phosphorylated by the growth associated H1 kinase.
用[32P] - 磷酸盐对处于中期的HeLa细胞进行体内标记，并使用部分纯化的与生长相关的H1激酶对HMG I进行体外磷酸化，以研究HMG I的中期特异性磷酸化。结果发现苏氨酸53和78发生了磷酸化。这些氨基酸分别嵌入序列PTPKR和TPGRK中，这两个序列与被生长相关的H1激酶磷酸化的序列相似。
