Department of Chemical Engineering, University of Coimbra, Polo II, Pinhal de Marrocos, 3030-790 Coimbra, Portugal.
J Biomed Mater Res A. 2011 Sep 15;98(4):535-43. doi: 10.1002/jbm.a.33146. Epub 2011 Jun 16.
Depending on the final application envisaged for a given biomaterial, many surfaces must be modified before use. The material performance in a biological environment is mainly mediated by its surface properties that can be improved using suitable modification methods. The aim of this work was to coat poly(dimethyl siloxane) (PDMS) surfaces with biosurfactants (BSs) and to evaluate how these compounds affect the PDMS surface properties. BSs isolated from four probiotic strains (Lactococcus lactis, Lactobacillus paracasei, Streptococcus thermophilus A, and Streptococcus thermophilus B) were used. Bare PDMS and PDMS coated with BSs were characterized by contact angle measurements, infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The influence of the surface modifications on the materials blood compatibility was studied through thrombosis and hemolysis assays. The cytotoxicity of these materials was tested against rat peritoneal macrophages. AFM results demonstrated the successful coating of the surfaces. Also, by contact angle measurements, an increase of the coated surfaces hydrophilicity was seen. Furthermore, XPS analysis indicated a decrease of the silicon content at the surface, and ATR-FTIR results showed the presence of BS characteristic groups as a consequence of the modification. All the studied materials revealed no toxicity and were found to be nonhemolytic. The proposed approach for the modification of PDMS surfaces was found to be effective and opens new possibilities for the application of these surfaces in the biomedical field.
根据给定生物材料的最终应用设想，许多表面在使用前必须进行改性。生物环境中的材料性能主要由其表面性能介导，这些性能可以通过合适的改性方法来改善。本工作的目的是在聚二甲基硅氧烷（PDMS）表面涂覆生物表面活性剂（BSs），并评估这些化合物如何影响 PDMS 表面性能。使用了从四种益生菌菌株（乳球菌乳球菌、副干酪乳杆菌、嗜热链球菌 A 和嗜热链球菌 B）中分离得到的 BSs。通过接触角测量、红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）和原子力显微镜（AFM）对裸 PDMS 和涂覆 BS 的 PDMS 进行了表征。通过血栓形成和溶血试验研究了表面改性对材料血液相容性的影响。通过大鼠腹腔巨噬细胞测试了这些材料的细胞毒性。AFM 结果证明了表面的成功涂覆。此外，通过接触角测量，发现涂覆表面的亲水性增加。此外，XPS 分析表明表面的硅含量减少，ATR-FTIR 结果表明由于改性而存在 BS 特征基团。所有研究的材料均无毒性且无溶血作用。发现 PDMS 表面改性的这种方法是有效的，并为这些表面在生物医学领域的应用开辟了新的可能性。
