CEB - Centre of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
Department of Biology, CITAB - Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Minho, Braga 4710-057, Portugal.
AMB Express. 2014 Apr 15;4:40. doi: 10.1186/s13568-014-0040-0. eCollection 2014.
Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (BioEG) produced by Lactobacillus paracasei subsp. paracasei A20 was evaluated. Both biosurfactants were tested against two breast cancer cell lines, T47D and MDA-MB-231, and a non-tumour fibroblast cell line (MC-3 T3-E1), specifically regarding cell viability and proliferation. Surfactin was found to decrease viability of both breast cancer cell lines studied. A 24 h exposure to 0.05 g l(-1) surfactin led to inhibition of cell proliferation as shown by cell cycle arrest at G1 phase. Similarly, exposure of cells to 0.15 g l(-1) BioEG for 48 h decreased cancer cells' viability, without affecting normal fibroblasts. Moreover, BioEG induced the cell cycle arrest at G1 for both breast cancer cell lines. The biosurfactant BioEG was shown to be more active than surfactin against the studied breast cancer cells. The results gathered in this work are very promising regarding the biosurfactants potential for breast cancer treatment and encourage further work with the BioEG glycoprotein.
生物表面活性剂是由微生物产生的具有表面活性的分子，可用于许多生物医学应用。这些分子的抗肿瘤潜力正在研究中，尽管结果仍然很少，并且关于这种活性的机制几乎没有数据。在这项工作中，评估了枯草芽孢杆菌 573 产生的表面活性剂和副干酪乳杆菌亚种副干酪乳杆菌 A20 产生的糖蛋白（BioEG）的抗肿瘤活性。两种生物表面活性剂都针对两种乳腺癌细胞系（T47D 和 MDA-MB-231）和一种非肿瘤成纤维细胞系（MC-3T3-E1）进行了测试，特别是关于细胞活力和增殖。发现表面活性剂降低了两种研究的乳腺癌细胞系的活力。暴露于 0.05 g l(-1)表面活性剂 24 小时导致细胞周期停滞在 G1 期，从而抑制细胞增殖。同样，将细胞暴露于 0.15 g l(-1) BioEG 48 小时降低了癌细胞的活力，而不影响正常成纤维细胞。此外，BioEG 诱导两种乳腺癌细胞系的细胞周期停滞在 G1 期。生物表面活性剂 BioEG 对研究中的乳腺癌细胞的活性比表面活性剂更强。这项工作的结果对于生物表面活性剂在乳腺癌治疗中的潜力非常有希望，并鼓励进一步研究 BioEG 糖蛋白。
