Department of Chemical Engineering, University of Coimbra, Polo II, Pinhal de Marrocos, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.
Colloids Surf B Biointerfaces. 2010 Nov 1;81(1):20-6. doi: 10.1016/j.colsurfb.2010.06.014. Epub 2010 Jun 25.
Poly(dimethyl siloxane) elastomer, (PDMS) is widely used as a biomaterial. However, PDMS is very hydrophobic and easily colonized by several bacteria and yeasts. Consequently, surface modification has been used to improve its wettability and reduce bacterial adhesion. The aim of this work was to modify the PDMS surface in order to improve its hydrophilicity and bacterial cell repulsion to be used as a biomaterial. Plasma was used to activate the PDMS surface and sequentially promote the attachment of a synthetic surfactant, Pluronic F-68, or a polymer, Poly(ethylene glycol) methyl methacrylate, PEGMA. Bare PDMS, PDMS argon plasma activated, PDMS coated with Pluronic F-68 and PEGMA-grafted PDMS were characterized by contact angle measurements, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The influence of the surface modifications on blood compatibility of the materials was evaluated by thrombosis and haemolysis assays. The cytotoxicity of these materials was tested for mouse macrophages. After modification, AFM results suggest the presence of a distinct layer at the surface and by the contact angle measures it was observed an increase of hydrophilicity. XPS analysis indicates an increase of the oxygen content at the surface as a result of the modification. All the studied materials revealed no toxicity and were found to be non-haemolytic or in some cases slightly haemolytic. Therefore, plasma was found to be an effective technique for the PDMS surface modification.
聚二甲基硅氧烷（PDMS）弹性体广泛用作生物材料。然而，PDMS 非常疏水，容易被几种细菌和酵母定植。因此，表面改性已被用于改善其润湿性和减少细菌黏附。本工作的目的是改性 PDMS 表面以提高其亲水性和排斥细菌细胞，用作生物材料。等离子体用于激活 PDMS 表面，然后依次促进合成表面活性剂 Pluronic F-68 或聚合物聚乙二醇甲基丙烯酸酯（PEGMA）的附着。通过接触角测量、X 射线光电子能谱（XPS）和原子力显微镜（AFM）对裸 PDMS、氩等离子体处理的 PDMS、涂覆 Pluronic F-68 的 PDMS 和接枝 PEGMA 的 PDMS 进行了表征。通过血栓形成和溶血试验评估表面改性对材料血液相容性的影响。用小鼠巨噬细胞测试这些材料的细胞毒性。改性后，AFM 结果表明表面存在明显的层，通过接触角测量观察到亲水性增加。XPS 分析表明，由于改性，表面的氧含量增加。所有研究的材料均未显示出毒性，并且发现非溶血或在某些情况下轻微溶血。因此，等离子体被发现是 PDMS 表面改性的有效技术。
